Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA.
Fedoreyeva LI, Kireev II, Khavinson VKh et al. · Biochemistry. Biokhimiia · PMID 22117547
Study summary
Main finding
Labeled Pinealon and other short peptides entered cells and nuclei; oligonucleotide assays indicated sequence-dependent interactions.
Fluorescently labeled peptides in HeLa cells and DNA assays
How the study was set up
Fluorescently labeled short peptides were examined in HeLa cells, with separate assays using DNA-related structures.
What the researchers measured
Cellular localization and fluorescence changes during interactions with different nucleic-acid sequences were measured.
How to read this evidence
Researchers studied cells, isolated tissue, or animals. These experiments can help explain a mechanism, but do not establish benefits or safety in people. Human cells grown in a laboratory also belong in this category.
Results in context
The binding and localization results suggest molecular interactions. They do not establish beneficial gene regulation, safety, or a neurological effect in people.
What this does not establish
Fluorescently labeled peptides and a cancer-cell line do not establish human gene regulation, therapeutic epigenetic effects, or safety.
Publication & source
Why this paper is included
The study tested HeLa-cell uptake of fluorescently labeled Pinealon and separate in vitro peptide-DNA interactions.
Publication indexing
Journal Article · Research Support, Non-U.S. Gov't